2010, Volume 6, Issue 4

Morphometric effect of nandrolone decanoate used as doping in sport on femur of rats in puberty period



Sefa Lök1, Hakan Yalcin2

1Physical Education and Sports Academy, Konya, Turkey
2Department of Anatomy, Veterinary Faculty, Selcuk University, Konya, Turkey


Author for correspondence: Sefa Lök; Physical Education and Sports Academy, Konya, Turkey; email: sefalok[at]selcuk.edu.tr


Full text

Abstract

Background and Study Aim: The aim of this research is to show the morphometric effect of nandrolone that is used as doping in sport on femur of rats in puberty period.
Material and Methods: A total of 60 Sprague–Dawley rats (30 days old) were divided into three equal groups. The control group (male n=10, female n=10) was nourished for 4 weeks without any substance being administered. For the peanut oil group (male n=10, female n=10), peanut oil was used as diluent of nandrolone decanoate (500 mcl). It was administered for 5 days via intraperitoneal injection, with a pause of 2 days and the same application was done for 4 weeks.. Nandrolone decanoate was applied for 5 days to the Nandrolone group (male n=10, female n=10) in 10 mg/kg dose by being diluted in 500 mcl peanut oil via intraperitoneal injection and the same was applied for 4 weeks having taken a 2-day long pause. Rats were euthanatized at the end of fourth week. Back extremity bones of materials were revealed by being dissected and put to maceration operation.
Results: Morphometric measures were taken by determining the anatomic reference points (length, corpus, cortex-cortical bone thickness-substantiate compact, medullar wide-cavity medullar) that would be measured of femur bones that are on right side. It was determined that nandrolone caused shortening in femur length (p<0.05) in male and female rats when compared to peanut oil and control groups.
Conclusions: Nandrolone may delay the bone formation in the puberty period.


Key words: femur, morphometric, nandrolone